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Q1: What is the difference between using NS0 or CHO cells for preparation of antibodies?
Answer
Q2: Is Lonza recommending that customers use CHO cell lines instead of NS0 cell lines for protein expression?
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Q3: Why use a Kozak sequence?
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Q4: Does Lonza recommend a specific leader sequence for optimum expression?
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Q5: Should I amplify GS-NS0 cell lines?
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Q6: Why are the plates left undisturbed for 3 weeks after transfection (isn’t there a risk of the medium evaporating, will the cells be in good condition, etc.)?
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Q7: Should I use dialysed foetal calf serum for transfections?
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Q8: What can I expect to see in a well on a 96 well plate after transfecting the NS0 host cells?
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Q9: What’s the difference between the pEE12.4 and pEE6.4 vectors?
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Q10: Why does Lonza recommend constructing a double gene vector (DGV) before transfection?
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Q11: What can I expect to see in a well on a 96 well plate after transfecting the CHOK1SV host cells?
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Q12: How do I submit questions or comments?
Answer: Please email us directly at GSLonza@lonza.com to submit questions and comments.
Q13: I have published a paper using GS. How do I get this information to Lonza?
Answer: Please forward all relevant details to us via email. It will be added to the GS Bibliography.
Q14: Where can I view the GS Bibliography?
Answer: The GS Bibliography can be read here. |
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