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What is the difference between using NS0 or CHO cells for preparation of antibodies? |
| The glycosylation of these two cell lines is different. It is generally thought that the glycosylation patterns of proteins from CHO cells are closer to those in humans. However, as antibodies are not heavily glycosylated, the glycosylation pattern tends to be less of an issue than with other types of products. There have been a number of reports of NS0 glycosylation affecting function when compared to the same product expressed from CHO cells. If the glycosylation pattern of the antibody is known to be important to its function then a comparison of the product produced both in CHO and NS0 cells may be required. The antibody products prepared for clinical trials at Lonza are expressed in either NS0 or CHO cells. There are currently four licensed products that are produced using GS-NS0 cell lines. Relevant references are included below.
Bebbington C.R. (1991): Expression of antibody genes in non-lymphoid mammalian cells. In Methods: A Companion to Methods in Enzymology 2: 136-145.
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Lifely, M.R., Hale, C., Boyce, S., Keen, M.J. and Phillips, J. (1995). Glycosylation and biological activity of Campath - IH expressed in different cell lines and grown under different culture conditions. Glycobiology 5, 813-822.
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Robinson, D.K., Chan, C.P., Yu Ip, C.C., Seamans, T.C., Lee, D.K., Lenny, A.B., Tung, J.-S., Di Stefano, D.J., Munshi, S., Gould, S.L., Tsai, P.K., Irwin, J., Mark, G.E. and Silberklang, M. (1994). Product consistency during long-term fed-batch culture. In Animal Cell Technology: Products of Today, Prospects for Tomorrow. Butterworths-Heinemann, pp763-767.
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Robinson, D.K., Chan, C.P., Yu Ip, C., Tsai, P.K., Tung, J., Seamans, T.C., Lenny, A.B., Lee, D.K., Irwin, J. And Silberklang, M. (1994). Characterization of a Recombinant Antibody Produced in the Course of a High Yield Fed-Batch Process. Biotechnology and Bioengineering 44, 727-735.
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Yu Ip, C.C., Miller, W.J., Silberklang, M., Mark, G.E., Ellis, R.W., Huang, L., Glushka, J., Van Halbeek, H., Zhu, J and Alhadeff, J.A. (1994). Structural characterisation of the N-Glycans of a humanised Anti-CD18 Murine Immunoglobulin G. Arch Biochem Biophys 308, 387 – 399.
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